However, interpreting the Widal test is important to arrive at the correct results. This test helps detect life-threatening illnesses like typhoid fever. It looks for O and H antibodies in a patient’s sample blood (serum). The Widal test is an advanced way to check for antibodies that your body makes against the salmonella bacteria that causes typhoid fever. This test was first conducted in 1896 by Georges Ferdinand Widal and was named after him. A Widal test meaning is a sersology blood test that helps detect typhoid or enteric fever in the body. For more information about Rapid tests, CLIA tests, IFA kits, or ELISA test kits, please contact our Customer Service Representatives at 81. also offers other Infectious Diseases Rapid tests, and Infectious Diseases ELISA test kits. See Salmonella Typhi Rapid Test Product Insert for specific details on preparation, procedures, quality control, and interpretation of test results.ĭiagnostic Automation Inc. Rapid and accurate test results within 10-20 minutes Simple and safe specimen collection directionsĬomprehensive package of required materials and components Notable features of Salmonella Rapid Test:Ĭonvenient and easy-to-understand directions For additional details please refer to the instructions for use. This forms a visible pink/purple band in the test line zone area (T). typhi, the antibody captures the complex. The complex continue to migrate along the membrane to the test band region where S.typhi specific LPS antibody is immobilized. typhi antigen present in the sample binds to the conjugate forming an antigen/antibody complex. As the specimen flows through the absorbent pad in the sample well and through the antibody/colloidal gold conjugate S. typhi (typhoid) infection with a high degree of sensitivity and specificity. typhi lipopolysaccharide (LPS) to selectively identify S. The test employs antibodies specific to S. Typhi Antigen RapiCard InstaTest is a qualitative one step immunochromatographic assay. It is the easiest and most specific method for detecting S. Typhi Antigen RapiCard InstaTest takes only 10-20 minutes and requires only a small quantity of stool or one drop of serum to perform. Other tests include the Widal reaction which lacks both sensitivity and specificity. The isolation of the bacilli is very time consuming and antibody detection is not very specific. The diagnosis of typhoid consists of isolation of the bacilli and the demonstration of antibodies. Towards the end of the incubation period, there occurs a massive bacteremia from these sites, heralding the onset of the clinical symptoms. A transient bacteremia follows, during which the bacilli are seeded in the liver, gall bladder, spleen, bone marrow, lymph nodes and kidneys, where further multiplication takes place. They enter the mesenteric lymph nodes, where they multiply and, via the thoracic duct, enter the blood stream. The ability to resist intracellular killing and to multiply within these cells is a measure of their virulence. They are then phagocytosed there by polymorphs and macrophages. On reaching the gut, the bacilli attach themselves to the epithelial cells of the intestinal villi and penetrate to the lamina and submucosa. The infection is acquired typically by ingestion. It is common in developing countries where it affects about 12.5 million persons annually.
Typhoid fever is a life threatening illness caused by the bacterium Salmonella Typhi, and was observed by Eberth (1880) in the mesenteric nodes and spleen of fatal cases of typhoid fever. Salmonella Rapid Test Background Information Materials Provided with Salmonella Rapid Test kit:Įach cassette contains a test strip with Salmonella Typhi specific antibody The Salmonella Rapid Test results are intended to help in the diagnosis of Salmonella Typhi infection and to monitor the effectiveness of therapeutic treatment.
One Step Salmonella Typhi Antigen RapiCard Insta Test cassette is a qualitative immunochromatographic assay for the rapid detection of Salmonella Typhi antigens in human stool or whole blood/plasma/serum specimen.
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